Screening Assays

Addex is the first company that has developed high-throughput industrial discovery tools for allosteric modulators. Addex has developed proprietary real time kinetic and proximal detection systems that permit the identification of allosteric modulators which modify the molecular response of their target. 

The company’s approach to leveraging and maintaining its competitive advantage is to keep certain aspects of its platform technology as trade secrets while patenting others. 

Proprietary screening assays developed at Addex include ProxyLiteTMand AddeLiteTM. These assays are used for screening GPCR and non-GPCR drug targets and are necessary to facilitate medicinal chemistry for lead optimization. 

ProxyLiteTM assay for GPCRs is a proprietary assay applicable to all GPCRs, family A through C, Gi-, Gs- or Gq-coupled. Signal measurement is dynamic and real-time, allowing kinetic analyses of activation and change induced by allosteric modulators. The overall result is a truer readout of receptor activation, allowing a better detection of allosteric modulators of GPCRs. 

AddeLiteTM assay for non-GPCRs is a proprietary assay applicable to all single-pass transmembrane proteins. It offers a unique way of measuring dynamically the activation of receptors of interest which have historically been difficult to address with small molecules. AddeLiteTM assay is highly appropriate for high-throughput screening, offering an exquisite sensitivity to detect allosteric modulators.